Part:BBa_K256003

J23119:GFP

GFP Reporter

This part constitutively expresses GFP.

Usage and Biology

Part J23119 is a member of the family of constitutive promoter parts. It allows strong expression of GFP.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 705
  

Applications of BBa_K256003

This part constitutively expresses GFP. Since J23119 was used as the constitutive promoter for NorR producing construct( BBa_K256007), it is necessary for us to understand its modular function and how it would affect NorR production.

Characterization Data

Characterization of J23119

Preliminary characterisation of J23119 showed that the activity of the promoter to be the strongest at 25°C and weakest at 37°C with 30°C being something of an intermediate. Activity of the J23119 was independent of time. Noteworthy was that there was a drastic drop in activity of the J23119 promoter at 16min, 37°C.

Average GFP/OD of J23119-GFP in different medium

It shows that the supplemented M9 medium is the ideal choice for the characterization of the J23119 promoter. M9 is most likely to be the ideal medium for characterisation of other promoters in general.

Average GFP/OD of J23119-GFP in steady state (M9)

Vector backbone did not seem to have much of an effect on the strengths of J23119, although pSB1A2 has a slight positive effect on the activity of the J23119.

Comparison of J23119 with other promoters

Based on our preliminary characterization results, it appeared that J23101 produced a much stronger signal than J23119 and pTet. This was quite surprising because J23119 promoter is supposed to be stronger then the J23101.

Characterization of J23119 in different chassis

J23119 performance appeared to be the best in Origami B.